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The 'LightMix ® Kit HFE' is sold under license from Roche Diagnostics and Bio-Rad Laboratories (worldwide except USA). LightCycler ® is a trademark from Roche. Homogenous amplification methods with real-time detection are covered by patents owned by Roche Molecular Systems, Inc. and F. Hoffmann-La Roche Ltd. Use of these methods requires a license. Mutations in the HFE gene are responsible for more than 90% of all hemochromatosis cases. Our LightMix ® Kit HFE detects the most impor tant mutation Cys 282 Tyr as well as His 63 Asp and Ser 65 Cys. This in-vitro diagnostic test is based on one dual color duplex PCR reaction, using the fluorescent probe based melting analysis method. Order number 40-0340 LightMix ® Kit HFE 282/63/65 The LightMix ® Kit HFE has been developed to work with the LightCycler ® Instruments 1.2/1.5, 2.0 and 480 using the channels 530 (F1) and 640 (F2). The kit contains the Roche Diagnostics LightCycler ® FastStart DNA Master. Using the kit requires to run the 530/640 color compensation once [order no. 40-0318-00]. Iron Overload? Molecular test for hemochromatosis risk DEUTSCHLAND TIB MOLBIOLGmbH Email: dna@tib-molbiol.de Tel. +49 30 78 79 94 55 Fax +49 30 78 79 94 99 USA TIB MOLBIOL LLC Email: dna@tibmolbiol.com Tel. +1 (877) 696-5446 Fax +1 (877) 696-5456 WWW. T I B -MOLB IOL . COM ESPAÑA TIB MOLBIOL sl Email: dna@tib-molbiol.es Tel. +34 91 344 6642 Fax +34 91 344 6670 ITALIA TIB MOLBIOL s.r.l. Email: dna@tibmolbiol.it Tel. +39 010 362 83 88 Fax +39 010 362 19 38 I SO 9 0 0 1 ISO 13485 C E R T I F I E D C O M P A N Y IVD